Guest house with restaurant, Plumlov

Předmětem diplomové práce je stavba penzionu s restaurací v obci Plumlov u Prostějova. Jedná se o budovu s podlažím částečně zapuštěným do terénu a s dvěma nadzemními podlažími. Suterénní část je navržena jako restaurace. Nadzemní podlaží jsou navrženy pro potřeby penzionu. V penzionu se nachází celkem 6 ubytovacích pokojů s celkovou kapacitou 15 osob. V restauraci je prostor pro pohodlné stolování cca 25 osob. K restauraci patří také kuchyně, kde se budou připravovat teplé pokrmy. Obvodové konstrukce podzemního podlaží jsou ze ztraceného bednění a nadzemní podlaží jsou řešeny jako dřevostavba systémem „two by four“. Strop nad restaurací je řešen jako systémový, železobetonový, skládaný (systém TRAS). Strop nad 1NP je řešen pomocí dřevěných stropních trámů. Střecha je sedlová s nesymetrickými sklony. Objekt je založen na základových pasech.The subject of this master´s thesis is the construction of the guest house with restaurant in the village Plumlov near Prostejov. It is a building with a floor partially sunk into the ground and two floors. Basement is designed as part of the restaurant. Floors are designed for the guest house. In the pension there is a total of 6 accommodation rooms with a total capacity of 15 persons. The restaurant has a comfortable dining area for 25 people. The restaurant also includes a kitchen where they will prepare hot meals. Cladding basement of the shuttering and floors are designed as wood house by system "two by four". The ceiling above the restaurant is designed as a system, reinforced concrete, folded (TRAS system). The ceiling above the 1st floor is designed with wooden ceiling beams. The roof is gabled with unbalanced tendencies. The building is based on the foundation strips.

Cryopreservation of Abies alba embryogenic tissues by slow-freezing method

Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated with 5% DMSO. Subsequently, the tissues were frozen at a cooling rate of 1 °C min-1 to -40 °C and transferred to liquid nitrogen for 72 hours. After thawing in a water bath at 40 °C, the tissues were cultivated on a proliferation medium. All tested lines recovered, but variations in regrowth frequencies across cell lines were noticed (91.66 to 100%). The recovered tissues showed similar features to the control 2 (non-pre-treated and non-cryopreserved tissues). In the accumulation of fresh and dry mass, no statistically significant differences were observed between cryopreserved cultures and control 2. The cryopreserved tissues produced cotyledonary somatic embryos capable of germination. Microscopic observations revealed considerable structural changes as a consequence of the cryopreservation procedure. The long vacuolated suspensor cells were disrupted, and mostly the meristematic cells of the embryonal region survived. The typical bipolar structure of early somatic embryos has been regained during the post-thaw period. Differences in cryotolerance across cell lines were also observed

Cryopreservation of Abies alba embryogenic tissues by slow-freezing method

Embryogenic tissues of Abies alba Mill. were cryopreserved using the slow-freezing approach. Four cell lines were incubated for 24 h on a medium with 0.5 M sorbitol and pre-treated with 5% DMSO. Subsequently, the tissues were frozen at a cooling rate of 1 °C min-1 to -40 °C and transferred to liquid nitrogen for 72 hours. After thawing in a water bath at 40 °C, the tissues were cultivated on a proliferation medium. All tested lines recovered, but variations in regrowth frequencies across cell lines were noticed (91.66 to 100%). The recovered tissues showed similar features to the control 2 (non-pre-treated and non-cryopreserved tissues). In the accumulation of fresh and dry mass, no statistically significant differences were observed between cryopreserved cultures and control 2. The cryopreserved tissues produced cotyledonary somatic embryos capable of germination. Microscopic observations revealed considerable structural changes as a consequence of the cryopreservation procedure. The long vacuolated suspensor cells were disrupted, and mostly the meristematic cells of the embryonal region survived. The typical bipolar structure of early somatic embryos has been regained during the post-thaw period. Differences in cryotolerance across cell lines were also observed

Physiological and structural aspects of in vitro somatic embryogenesis in Abies alba Mill

Initiation of somatic embryogenesis fromimmature zygotic embryos, long-term maintenance of embryogenic tissue in vitro or by cryopreservation, as well as maturation, of somatic embryos of Abies alba Mill. are reported in this study. For the initiation of embryogenic tissues, a DCR medium containing di erent types of cytokinins (1 mg.L1) were tested. During three consecutive years, 61 cell lines were initiated out of 1308 explants, with initiation frequencies ranging between 0.83 and 13.33%. The type of cytokinin had no profound e ect on the initiation frequency within one given year. Microscopic observations revealed presence of bipolar somatic embryos in all initiated embryogenic tissues. Besides the typical bipolar somatic embryos, huge polyembryonal complexes, as well as “twin” embryos, were observed. Maturation of somatic embryos occurred on a DCR medium supplemented by abscisic acid (10 mg.L1), polyethylene glycol (PEG-4000, 7.5%) and 3% maltose. The maturation capacity was cell-line dependent. All of the four tested cell lines produced cotyledonary somatic embryos, though at di erent quantities, of 16 to 252 per g of fresh weight. After germination, seedlings developed, but their further growth soon stopped after the formation of a resting bud. Altogether, seven cell lines were cryopreserved, using the slow-freezing technique. After rewarming, all tested cell lines showed regrowth rates between 81.8 and 100%

Tissue regeneration of Abies embryogenic cell lines after 1 year storage in liquid nitrogen

© 2016 Institute of Botany, Slovak Academy of Sciences. Embryogenic tissues of hybrid firs (Abies alba × A. cephalonica, Abies alba × A. numidica) have been cryopreserved using a slow-freezing method. The cryotolerance of six cell lines initiated from immature or mature zygotic embryos was tested. Following sorbitol (0.5 M) and DMSO (5%) pretreatments the samples were slowly frozen at a rate of 1°C/min, plunged into liquid nitrogen and stored for 1 year. Post-thaw regeneration ocurred in all the six tested cell lines with recovery frequencies ranging from 100% (cell lines AC1, AC2, AC78, AN72), 90% (cell line AC2) to 44.4% (cell line AC79). Fresh and dry mass accumulation of cryopreserved tissues evaluated three month after thawing was identical to that of control (non-cryopreserved tissues without pretreatment). The cryopreservation procedure resulted in disintegration of bipolar structure of somatic embryos. The long vacuolised suspensor cells almost completely disrupted and the meristematic embryonal cells survived cryopreservation. In the post-thaw period, repeated cell divisions of meristematic cells led to formation of new cell clusters and their vacuolisation resulted in polarisation and finally to the formation of bipolar structures and somatic embryos.status: publishe

Physiological and Structural Aspects of In Vitro Somatic Embryogenesis in Abies alba Mill

Initiation of somatic embryogenesis from immature zygotic embryos, long-term maintenance of embryogenic tissue in vitro or by cryopreservation, as well as maturation, of somatic embryos of Abies alba Mill. are reported in this study. For the initiation of embryogenic tissues, a DCR medium containing different types of cytokinins (1 mg.L−1) were tested. During three consecutive years, 61 cell lines were initiated out of 1308 explants, with initiation frequencies ranging between 0.83 and 13.33%. The type of cytokinin had no profound effect on the initiation frequency within one given year. Microscopic observations revealed presence of bipolar somatic embryos in all initiated embryogenic tissues. Besides the typical bipolar somatic embryos, huge polyembryonal complexes, as well as “twin” embryos, were observed. Maturation of somatic embryos occurred on a DCR medium supplemented by abscisic acid (10 mg.L−1), polyethylene glycol (PEG-4000, 7.5%) and 3% maltose. The maturation capacity was cell-line dependent. All of the four tested cell lines produced cotyledonary somatic embryos, though at different quantities, of 16 to 252 per g of fresh weight. After germination, seedlings developed, but their further growth soon stopped after the formation of a resting bud. Altogether, seven cell lines were cryopreserved, using the slow-freezing technique. After rewarming, all tested cell lines showed regrowth rates between 81.8 and 100%status: publishe

High expression of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE coincides with initiation of various developmental pathways in in vitro culture of Trifolium nigrescens

The aim of this study was to identify and examine the expression pattern of the ortholog of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE gene from Trifolium nigrescens (TnSERK) in embryogenic and non-regenerative cultures of immature cotyledonary-stage zygotic embryos (CsZEs). In the presence of 1-naphthaleneacetic acid and N(6)-[2-isopentenyl]-adenine, the CsZE regenerated embryoids directly and in a lengthy culture produced callus which was embryogenic or remained non-regenerative. As revealed by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), the TnSERK was expressed in both embryogenic and non-regenerative cultures, but the expression level was significantly higher in embryogenic ones. An in situ RNA hybridization assay revealed that the expression of TnSERK preceded the induction of cell division in explants, and then, it was maintained exclusively in actively dividing cells from which embryoids, embryo-like structures (ELSs), callus or tracheary elements were produced. However, the cells involved in different morphogenic events differed in intensity of hybridization signal which was the highest in embryogenic cells. The TnSERK was up-regulated during the development of embryoids, but in cotyledonary embryos, it was preferentially expressed in the regions of the apical meristems. The occurrence of morphological and anatomical abnormalities in embryoid development was preceded by a decline in TnSERK expression, and this coincided with the parenchymatization of the ground tissue in developing ELSs. TnSERK was also down-regulated during the maturation of parenchyma and xylem elements in CsZE and callus. Altogether, these data suggest the involvement of TnSERK in the induction of various developmental programs related to differentiation/transdifferentiation and totipotent state of cell(s). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00709-015-0814-5) contains supplementary material, which is available to authorized users